Anti-NDUFB8 Antibody [ARC2259] (A307077)

Name: Anti-NDUFB8 Antibody [ARC2259]
Brand: Antibodies.com
SKU: A307077
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC2259] antibody to NDUFB8 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-NDUFB8 Antibody [ARC2259]

Description

Rabbit monoclonal [ARC2259] antibody to NDUFB8.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:3,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFB8 (O95169).

Sequence

MAVARAGVLGVQWLQRASRNVMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTSPTPVSWHVMCMQLFGFLAFMIFMCWVGDVY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2259

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CI-ASHI, Complex I-ASHI, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial, NADH-ubiquinone oxidoreductase ASHI subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-NDUFB8 Antibody [ARC2259] (A307077)

Western blot analysis of extracts of various cell lines, using Anti-NDUFB8 Antibody [ARC2259] (A307077) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-NDUFB8 Antibody [ARC2259] (A307077)

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Immunohistochemistry - Anti-NDUFB8 Antibody [ARC2259] (A307077)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-NDUFB8 Antibody [ARC2259] (A307077) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-NDUFB8 Antibody [ARC2259] (A307077)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NDUFB8 Antibody [ARC2259] (A307077) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.