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Anti-NDUFB4 Anticorpo (A92828)

Nome
Anti-NDUFB4 Antibody
Descrizione
Rabbit polyclonal antibody to NDUFB4.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Sequenza
MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQLKREYLLQYNDPNRRGLIENPALLRWAYARTINVYPNFRPTPKNSLMG
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
15 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
B15, CI-B15, Complex I B15 subunit, Complex I-B15, NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NADH ubiquinone oxidoreductase B15 subunit, NADH-ubiquinone oxidoreductase B15 subunit, NDUB4_HUMAN
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-NDUFB4 Antibody (A92828) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-NDUFB4 Antibody (A92828)
Western blot analysis of extracts of various cell lines, using Anti-NDUFB4 Antibody (A92828) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-NDUFB4 Antibody (A92828) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-NDUFB4 Antibody (A92828)
Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using Anti-NDUFB4 Antibody (A92828) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-NDUFB4 Antibody (A92828)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-NDUFB4 Antibody (A92828) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-NDUFB4 Antibody (A92828) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-NDUFB4 Antibody (A92828)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NDUFB4 Antibody (A92828) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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