Figure 1: Western Blot - Anti-NDUFA4 Antibody (A307518)
Western blot analysis of extracts from wild type(WT) and NDUFA4 knockout (KO) 293T(KO) cells, using Anti-NDUFA4 Antibody (A307518) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-NDUFA4 Antibody (A307518) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NDUFA4 Antibody (A307518) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-NDUFA4 Antibody (A307518) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using Anti-NDUFA4 Antibody (A307518) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-NDUFA4 Antibody (A307518) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
