Anti-NCKAP1 Anticorpo (A326266)

Name: Anti-NCKAP1 Antibody
Brand: Antibodies.com
SKU: A326266
Price: 448.0 USD
Availability: InStock

Clicca sull'immagine per la visualizzazione estesa

$448

Anticorpo policlonale di capra contro NCKAP1 per ELISA, IF e Flow Cytometry.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Descrizione Prodotto

Nome

Anti-NCKAP1 Antibody

Descrizione

Goat polyclonal antibody to NCKAP1.

Applicazioni

ELISA, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 0.5µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human NCKAP1.

Sequenza

C-TPREYLTSHLEIR

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

100 kDa

MW previsto

129kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

FLJ11291, KIAA0587, Membrane associated protein HEM2, Membrane-associated protein HEM-2, MGC8981, NAP 1, NAP1, NAP125, NCK associated protein, NCK associated protein 1, Nck-associated protein 1, NCKP1_HUMAN, OTTHUMP00000163460, OTTHUMP00000163461, p125Nap1

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Immunofluorescence - Anti-NCKAP1 Antibody (A326266)

NCKAP1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NCKAP1 Antibody (A326266) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Immunofluorescence - Anti-NCKAP1 Antibody (A326266)

NCKAP1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NCKAP1 Antibody (A326266) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Flow Cytometry - Anti-NCKAP1 Antibody (A326266)

NCKAP1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NCKAP1 Antibody (A326266) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.