Anti-Myosin Phosphatase (phospho Ser507) Anticorpo (A88181)

Western blot analysis of extracts of various cell lines, using Anti-Myosin Phosphatase (phospho Ser507) Antibody (A88181) at 1:2,000 dilution or PPP1R12A antibody (A0587). HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.