Anti-Myeloperoxidase Antibody - BSA and Azide free
Descrizione
Rabbit polyclonal antibody to Myeloperoxidase.
Specificità
The heme protein myeloperoxidase (MPO) is a major component of azurophilicgranules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme forthe generation of hypochlorous acid and other toxic oxygen products. TheMPO precursor is synthesized during the promyelocytic stage of myeloid dif-ferentiation and is subsequently processed and transported intracellularly tothe lysosomes. The precursor undergoes cotranslational N-linked glycosyla-tion to produce a glycoprotein. Glucosidases in the endoplasmic reticulum(ER) or early cisGolgi convert the pro-MPO to a form which is sorted into aprelysosomal compartment, which undergoes final proteolytic maturation tonative MPO, a pair of heavy-light protomers. In normal neutrophils, MPO isexpressed as a dimer. Calreticulin, a calcium-binding protein residing in theER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA isabundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60cells. MPO is expressed at high levels in circulating neutrophils and mono-cytes but is not detectable in microglia, brain-specific macrophages or normalbrain tissue.MPO, which has a molecular weight of approximately 140 kD, is homodimer that can be split into two halves that still have enzymatic activity. These hemi-MPOmonomersconsist of a 59-kD alpha chainand a 13.5-kD beta chain.
Applicazioni
WB, IHC-P
Diluizioni consigliate
WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml
Reattività
Human
Immunogeno
Synthetic peptide corresponding to amino acids 150-250 of Myeloperoxidase.
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
1 mg/ml
Peso molecolare
heavy-light promoter: 72 kDa / dimer: 140 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.