Recombinant mouse monoclonal [r1032] antibody to MVP.
Specificità
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Applicazioni
WB, IHC-P
Controllo positivo
MCF-7 or HeLa cells. Human breast tumors.
Diluizioni consigliate
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reattività
Human
Immunogeno
Proteins precipitated from human breast cancer MCF-7 cells.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
r1032
Isotipo
IgG1
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
104-110 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
LRP, Lung resistance related protein, Lung resistance-related protein, Major vault protein, Major vault protein, rat, homolog of, MVP_HUMAN, testicular secretory protein Li 30, VAULT 1, VAULT1