This mTOR (phospho Ser2481) Cell Based ELISA Kit allows for the detection of mTOR (phospho Ser2481) and the effects that certain stimulation conditions have on mTOR (phospho Ser2481) expression in different cell lines. Qualitative determination of mTOR (phospho Ser2481) concentration is achieved by an indirect ELISA format. In essence, the mTOR (phospho Ser2481) is captured by Anti-mTOR (phospho Ser2481) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this mTOR (phospho Ser2481) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-mTOR Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Adherent cells and suspension cells.

Campo di rilevamento

> 5000 Cells

Tempo del kit

4 hours 30 minutes

Reattività

Human, Mouse, Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at + 4°C. Stable for 6 months.

Sinonimi

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1), FK506 binding protein 12 rapamycin associated protein 1, FK506 binding protein 12 rapamycin associated protein 2, FK506 binding protein 12 rapamycin complex associated protein 1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, MTOR_HUMAN, OTTHUMP00000001983, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin associated protein FRAP2, Rapamycin target protein, Rapamycin target protein 1, RAPT1, Serine/threonine-protein kinase mTOR

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.