Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.
Applicazioni
WB, IHC-P
Controllo positivo
A549 or HepG2 cells. Human colon, thyroid, testis or lymph node.
Diluizioni consigliate
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reattività
Human
Immunogeno
Recombinant fragment (around Amino Acids 300-500) of human MSH2 protein. The exact sequence is proprietary.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
MSH2/3165
Isotipo
IgG2
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
100 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
BAT26, COCA 1, COCA1, DNA mismatch repair protein Msh2, FCC 1, FCC1, hMSH2, HNPCC, HNPCC 1, HNPCC1, LCFS2, MSH 2, MSH2_HUMAN, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2