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Anti-DOT1L Antibody (A12763)

Western Blot - Anti-MRPL39 Antibody (A10023) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to DOT1L for WB, IHC and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-DOT1L Antibody
Description
Rabbit polyclonal antibody to DOT1L.
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human DOT1L (NP_115871.1).
Sequence
TDKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLSGPGLAPAASSAGGAASSAQTHRSFLGPFPPGPQFALGPMSLQANLGSVAGSSVLQSLFSS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
170kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DOT1-like protein, H3-K79-HMTase, Histone H3-K79 methyltransferase, Histone-lysine N-methyltransferase, H3 lysine-79 specific, KIAA1814, KMT4, Lysine N-methyltransferase 4
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-MRPL39 Antibody (A10023) - Antibodies.comEnlarge Image

Western Blot - Anti-DOT1L Antibody (A12763)

Western blot analysis of extracts of various cell lines, using MRPL39 antibody (A10023) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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