MRE11 (phospho Ser264) Cell Based Kit Elisa (A102191)

Nome
MRE11 (phospho Ser264) Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This MRE11 (phospho Ser264) Cell Based ELISA Kit allows for the detection of MRE11 (phospho Ser264) and the effects that certain stimulation conditions have on MRE11 (phospho Ser264) expression in different cell lines. Qualitative determination of MRE11 (phospho Ser264) concentration is achieved by an indirect ELISA format. In essence, the MRE11 (phospho Ser264) is captured by Anti-MRE11 (phospho Ser264) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MRE11 (phospho Ser264) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-MRE11 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
AT like disease, Ataxia telangiectasia disorder like, ATLD, DNA recombination and repair protein, Double strand break repair protein MRE11A, Double-strand break repair protein MRE11A, endo/exonuclease Mre11, HNGS1, meiotic recombination (S. cerevisiae) 11 homolog A, Meiotic recombination 11 homolog 1, Meiotic recombination 11 homolog A, meiotic recombination 11 homolog A (S. cerevisiae), MmMRE11A, Mre 11, MRE 11a, MRE 11b, MRE11 homolog 1, MRE11 homolog A, MRE11 homolog double strand break repair nuclease, MRE11 meiotic recombination 11 homolog A, MRE11 meiotic recombination 11 homolog A (S. cerevisiae), MRE11A, MRE11b, MRE11_HUMAN, OTTHUMP00000236830, OTTHUMP00000236831, OTTHUMP00000236832, OTTHUMP00000236833
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Prodotti alternativi a MRE11 (phospho Ser264) Cell Based Kit Elisa (A102191)

Inizio pagina