Topo Thrombospondin 1 Kit Elisa (A323245)

$595
Spese di spedizione
Tempi di consegna
7-10 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
Mouse Thrombospondin 1 ELISA Kit (Small Sample Volume)
Descrizione
Mouse Thrombospondin 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Thrombospondin 1 in serum, plasma, and other biological fluids.
Tipo di kit
Sandwich (quantitative)
Principio di kit
Mouse Thrombospondin 1 ELISA Kit (A323245) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse Thrombospondin 1 in serum, plasma, and other biological fluids. An antibody specific for Thrombospondin 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Thrombospondin 1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Thrombospondin 1 Antibody, which binds the captured Thrombospondin 1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Thrombospondin 1 captured in each well. The concentration of Thrombospondin 1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Piattaforma
Pre-coated Microplate (12 x 8 well strips)
Tipo di rilevamento
Colormetric
Instrument
Colorimetric Microplate Reader
Tipo di campione
Serum, plasma, and other biological fluids.
Sensibilità
4.69 pg/ml
Campo di rilevamento
7.81-500 pg/ml
Tempo del kit
3h 30m
Reattività
Mouse
Reattività incrociata
No significant cross-reactivity or interference between mouse Thrombospondin 1 and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 94% - 108% 101%
EDTA Plasma 8 93% - 106% 100%
Cell Culture Media 8 88% - 98% 93%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 85% - 99% 87% - 102% 82% - 92% 84% - 97%
EDTA Plasma (n=5) 83% - 99% 85% - 96% 88% - 100% 83% - 95%
Cell Culture Media (n=5) 95% - 109% 90% - 105% 95% - 110% 94% - 107%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Sinonimi
THBS1, TSP, Tsp1
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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