Mouse ST2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse ST2 in the samples. A monoclonal antibody specific for mouse ST2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for ST2, and ST2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ST2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

10.49 pg/ml

Campo di rilevamento

62.50 - 4000 pg/ml

Recupero

102% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant mouse ST2.

Reattività

Mouse

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

DER-4, DER4, FIT 1, Growth stimulation expressed, homolog of mouse growth stimulation-expressed, Il1rl1, IL33R, ILRL1_HUMAN, Interleukin 1 receptor like 1, interleukin 1 receptor related protein, Interleukin-1 receptor-like 1, Protein ST2, ST2L, ST2V, T1, T1 protein

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.