This Resistin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Resistin Antibody. Standards or samples are then added to the microtire plate wells and Resistin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Resistin present in the sample, Anti-Resistin Antibody (Biotin) is added to each well to "sandwich" the Resistin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Resistin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensibilità

< 2 pg/ml

Campo di rilevamento

31.2-2000 pg/ml

Reattività

Mouse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2-8°C for 4 months or at -20°C for 8 months.

Sinonimi

Adipose tissue specific secretory factor , Adipose tissue-specific secretory factor, ADSF, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 , C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, CG5403, Cysteine rich secreted protein A12 alpha like 2 , Cysteine rich secreted protein FIZZ3, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, dri, FIZZ 3, FIZZ3, Found in inflammatory zone 3, HXCP 1, HXCP1, MGC126603, MGC126609, PRO1199, Resistin delta2 , RETN, RETN 1, RETN1, RETN_HUMAN, RSTN, UNQ407 , XCP 1 , XCP1

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.