Mouse Phospholipase A2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Phospholipase A2 in serum, plasma, tissue homogenates or other biological fluids.

Tipo di kit

Sandwich (quantitative)

Principio di kit

Mouse Phospholipase A2 ELISA Kit (A7340) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Phospholipase A2 in serum, plasma, tissue homogenates or other biological fluids. An antibody specific for Phospholipase A2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Phospholipase A2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Phospholipase A2 Antibody, which binds the captured Phospholipase A2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Phospholipase A2 captured in each well. The concentration of Phospholipase A2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Piattaforma

Pre-coated Microplate (12 x 8 well strips)

Tipo di rilevamento

Colourmetric

Instrument

Colorimetric Microplate Reader

Tipo di campione

Serum, plasma, tissue homogenates or other biological fluids.

Sensibilità

5.7 pg/ml

Campo di rilevamento

15.6-1000 pg/ml

Tempo del kit

4h 30m

Reattività

Mouse

Recovery

Sample Type	n	Range	Average
Serum	5	83% - 100%	91%
EDTA Plasma	5	90% - 98%	94%
Heparin Plasma	5	90% - 99%	94%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	90-99%	82-108%	82-96%	92-103%
EDTA Plasma (n=5)	84-105%	88-94%	89-107%	72-106%
Heparin Plasma (n=5)	89-92%	88-108%	84-102%	90-98%

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Note generali

Information online is representative. Always refer to the Product Manual inside the kit.

Conservazione

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Sinonimi

Calcium dependent phospholipid binding protein, CPLA 2, cPLA2, cPLA2 alpha, cPLA2-alpha, Cytosolic phospholipase A2, Cytosolic phospholipase A2 group IVA, GIIC sPLA2, Group IIA phospholipase A2, Lysophospholipase, membrane associated, MGC126350, MOM1, Non-pancreatic secretory phospholipase A2, NPS-PLA2, OTTHUMP00000002786, PA24A_HUMAN, PA2GA_HUMAN, Phosphatidylcholine 2 acylhydrolase, Phosphatidylcholine 2-acylhydrolase, Phosphatidylcholine 2-acylhydrolase 2A, Phospholipase A2 group 4 A, Phospholipase A2 group IIA, Phospholipase A2 group IVA, Phospholipase A2 group IVA (cytosolic calcium dependent), Phospholipase A2, group IIA (platelets, synovial fluid), Phospholipase A2, group IVA (cytosolic, calcium-dependent), PhospholipaseA2, PLA2, PLA2B, PLA2G2A, PLA2G4, pla2g4a, PLA2L, PLA2S, PLAS1, RASF A, sPLA2

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Kit Components

Item	Quantity	Storage
Pre-Coated 96 Well Microplate	12 x 8 Well Strips	-20°C
Lyopholized Standard	2 Vials	-20°C
Detection Solution A	120μl	-20°C
Detection Solution B	120µl	-20°C
Wash Buffer (30X)	20ml	+4°C
Sample Dilution Buffer	45ml	-20°C
TMB Substrate	9ml	+4°C
Stop Solution	6ml	+4°C
Plate Sealers	5 Adhesive Strips	-

Required Materials

The following materials are not included in the kit, but are required to run this assay:

Microplate reader capable of measuring absorbance at 450nm ± 10nm.
Squirt bottle, multichannel pipette reservoir, or automated microplate washer.
Graph paper or computer software capable of generating logarithmic functions.
Test tubes and Eppendorf tubes to prepare standards and samples.
Multi- and single-channel pipettes and sterile pipette tips.
Deionized or distilled water.
Absorbent paper towels.
37°C incubator.

Important Notes

Sample concentrations should be estimated before being used in the assay and a proper dilution factor should be selected to make the diluted target protein concentration fall in the optimal detection range of this kit.
Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.
Samples should be brought to room temperature before starting the assay.

Bring all reagents and samples to room temperature before use.

We recommend that all standards and samples be assayed in duplicate.

Step 1

Prepare all reagents, samples, and working standards as directed in the previous sections.

Step 2

Determine the number of microplate strips required for the assay and insert them into the plate frame. Unused strips should be stored in a foil pouch at -20°C.

Step 3

Add 100μl of standard solutions to the standard wells. Note: A plate layout is provided to record standards and samples assayed.

Step 4

Add 100μl of samples to the sample wells.

Step 5

Cover with an adhesive plate sealer provided and incubate at 37°C for 2 hours.

Step 6

Remove the plate sealer and aspirate the liquid from the plate.

Step 7

Add 100μl of working Detection Solution A to each well.

Step 8

Cover with a new adhesive plate sealer and incubate at 37°C for 60 minutes.

Step 9

Remove the plate sealer, aspirate the liquid from the plate, and wash the plate three times with Wash Buffer. Soak wells with 300μl of Wash Buffer for 1-2 minutes each time. Note: Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Blot the plate onto paper towels or other absorbent material. Do not let the wells dry completely at any time!

Step 10

Add 100μl of working Detection Solution B to each well.

Step 11

Cover with a new adhesive plate sealer and incubate at 37°C for 60 minutes.

Step 12

Remove the plate sealer, aspirate the liquid from the plate, and wash the plate five times with Wash Buffer. Soak wells with 300μl of Wash Buffer for 1-2 minutes each time.

Step 13

Add 90μl of TMB Substrate to each well. Cover with a new plate sealer and incubate at 37°C in the dark for 15-25 minutes (do not exceed 30 minutes). Note: This incubation time is for reference only, the optimal reaction time should be determined by the end-user and can be shortened or extended according to the actual color change. The reaction may be terminated when a gradient is apparent in the standard wells.

Step 14

Add 50μl of Stop Solution to each well. The color in the wells should change from blue to yellow immediately. Note: If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.

Step 15

Determine the optical density (O.D. value) of each well immediately using a microplate reader set to 450 nm.