Topo Leptin Kit Elisa (A1596)

Mouse Leptin ELISA Kit

Sandwich (quantitative)

Mouse Leptin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Leptin in the samples. A monoclonal antibody specific for mouse Leptin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Leptin, and Leptin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Leptin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

102% (Average Recovery from Serum Samples).

This kit can assay both natural and recombinant mouse Leptin.

Mouse

This assay has shown no significant cross-reactivity.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2 - 8°C.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.