Topo IL1 beta Kit Elisa (A602)

$635
Spese di spedizione
Tempi di consegna
7-10 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
Mouse IL1 beta ELISA Kit
Tipo di kit
Sandwich (quantitative)
Principio di kit
This IL1 beta enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IL1 beta Antibody. Standards or samples are then added to the microtire plate wells and IL1 beta, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IL1 beta present in the sample, Anti-IL1 beta Antibody (HRP) is added to each well to "sandwich" the IL1 beta immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL1 beta will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Serum, plasma, urine, cell culture supernatants, and tissue samples.
Campo di rilevamento
5-100 pg/ml
Reattività
Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at 2-8°C for 6 months.
Sinonimi
Catabolin, H1, Hematopoietin 1 , IFN beta inducing factor , IL 1 , IL 1 beta , IL 1B, IL 1beta, IL-1 beta, IL1 , IL1B, IL1B_HUMAN, IL1F2 , Interleukin 1 beta , Interleukin 1 beta precursor , Interleukin-1 beta, LAF, OAF, Osteoclast activating factor , OTTHUMP00000162031, Preinterleukin 1 beta, Preinterleukin beta , Pro interleukin 1 beta
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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