Figure 1: Western Blot - Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin) (A121262)
Western blot of non-reduced (-) and reduced (+) mouse IgG2a? (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG2a Kappa Antibody [RM107] (Biotin). This antibody only reacts to non-reduced Mouse IgG2a?.
ELISA of mouse immunoglobulins shows Anti-Mouse IgG2a Kappa Antibody [RM107] reacts to the Fab region of mouse IgG2a?. There is no cross reactivity with IgG2a?, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG2a Kappa Antibody [RM107] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG2a?. The plate was coated with different amounts of mouse IgG2a?. A serial dilution of Anti-Mouse IgG2a Kappa Antibody [RM107] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.