Topo IgG2a (FITC) - Isotype Control (A282454)

Mouse IgG2a (FITC) - Isotype Control

Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Activated rat T-helper cells.

Mouse

Monoclonal

IgG2a

FITC

Excitation: 490nm, Emission: 525nm

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.This antibody may not be suitable for intracellular staining on some cell types.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.