Topo IgG2a (APC) - Isotype Control (A282187)

Mouse IgG2a (APC) - Isotype Control

Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Activated rat T-helper cells.

Mouse

Monoclonal

IgG2a

APC

Excitation: 645nm, Emission: 660nm

Affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.This antibody may not be suitable for intracellular staining on some cell types.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.