Topo IgG1 (PE) - Isotype Control (A282643)

Mouse IgG1 (PE) - Isotype Control

Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ separated cells or 100µl lysed whole peripheral blood.

Mouse

Monoclonal

IgG1

PE

Excitation: 565nm, Emission: 578nm

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.