Topo IgG1 - BSA and Azide free - Isotype Control (A284000)

Mouse IgG1 - BSA and Azide free - Isotype Control

Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human T lymphocytes.

Mouse

Monoclonal

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse IgG1 Negative Control antibody is suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES