ELISA of mouse immunoglobulins shows Anti-Mouse IgG F(ab) Antibody [RMG05] reacts to the Fab region of mouse IgG1, IgG2a, IgG2b, and IgG3. There is no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50ng/well of different immunoglobulins. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Mouse IgG F(ab) Antibody [RMG05] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of Anti-Mouse IgG F(ab) Antibody [RMG05] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.