Figure 1: Western Blot - Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin) (A121254)
Western blot of non-reduced (-) and reduced (+) mouse IgG1? and IgG1? (20ng/lane), using 0.2µg/ml of Anti-Mouse Ig Lambda Light Chain Antibody [RM110] (Biotin). This antibody reacts to non-reduced IgG1?.
ELISA of mouse immunoglobulins shows Anti-Mouse Ig Lambda Light Chain Antibody [RM110] reacts to the lambda light chain of mouse immunoglobulins. There is no cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse Ig Lambda Light Chain Antibody [RM110] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG1?. The plate was coated with different amounts of mouse IgG1?. A serial dilution of Anti-Mouse Ig Lambda Light Chain Antibody [RM110] was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.