Topo Hemoglobin Kit Elisa (A327044)

Mouse Hemoglobin ELISA Kit

Mouse Hemoglobin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of mouse Hemoglobin in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Mouse Hemoglobin ELISA Kit (A327044) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Hemoglobin in serum, plasma, and other biological fluids. An antibody specific for Hemoglobin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Hemoglobin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Hemoglobin Antibody, which binds the captured Hemoglobin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Hemoglobin captured in each well. The concentration of Hemoglobin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

93.75 ng/ml

156.25-10000 ng/ml

3h 30m

No significant cross-reactivity or interference between mouse Hemoglobin and analogues was observed.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

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