Mouse Granzyme B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Granzyme B in the samples. A monoclonal antibody specific for mouse Granzyme B has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Granzyme B, and Granzyme B present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Granzyme B bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

9.91 pg/ml

Campo di rilevamento

312.50 - 20000 pg/ml

Recupero

99% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant mouse Granzyme B.

Reattività

Mouse

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

C11, Cathepsin G like 1, Cathepsin G-like 1, CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA-1, CTLA1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T lymphocyte proteinase 2, Cytotoxic T-lymphocyte proteinase 2, Fragmentin 2, Fragmentin-2, GRAB_HUMAN, Granzyme 2, Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1), Granzyme-2, GranzymeB, GRB, Gzmb, Hlp, Human lymphocyte protein, Lymphocyte protease, Protease, serine, B, SECT, T cell serine protease 1-3E, T-cell serine protease 1-3E

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.