Principio di kit
Mouse gamma Endorphin ELISA Kit (A7328) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse gamma Endorphin in serum, plasma, tissue homogenates or other biological fluids. The 96-well microtiter plate has been pre-coated with gamma Endorphin antigen. During the incubation, gamma Endorphin present in the samples or standards competes with the fixed amount of immobilized gamma Endorphin for binding sites on the Biotinylated Anti-gamma Endorphin Antibody. The more gamma Endorphin present in a sample or standard, the less Biotinylated Anti-gamma Endorphin Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-gamma Endorphin Antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of gamma Endorphin present in each sample or standard. The concentration of gamma Endorphin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.