Topo Fetuin A Kit Elisa (A323239)

Mouse Fetuin A ELISA Kit (Small Sample Volume)

Mouse Fetuin A ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Fetuin A in serum, plasma, and other biological fluids. This ELISA kit only requires 25µl of sample volume(s).

Sandwich (quantitative)

Mouse Fetuin A ELISA Kit (A323239) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse Fetuin A in serum, plasma, and other biological fluids. An antibody specific for Fetuin A has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Fetuin A present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Fetuin A Antibody, which binds the captured Fetuin A present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Fetuin A captured in each well. The concentration of Fetuin A can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.03 µg/ml

0.08-5 µg/ml

Mouse

No significant cross-reactivity or interference between mouse Fetuin A and analogues was observed.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

59 kDa bone sialic acid containing protein, A2HS, Aa2 066, AHS, Ahsg, Alpha 2 HS glycoprotein, Alpha 2 HS glycoprotein chain A, Alpha 2 HS glycoprotein chain B, Alpha 2 Z globulin, Alpha 2HS glycoprotein, Alpha-2-HS-glycoprotein chain B, Alpha-2-Z-globulin, alpha2 Heremans Schmid glycoprotein, Asialofetuin, Ba alpha 2 glycoprotein, Ba-alpha-2-glycoprotein, BSP, Countertrypin, FETUA, FETUA_HUMAN, Fetuin, mouse, homolog of, Fetuin-A, FetuinA, Glycoprotein PP63, HSGA, pp63