Topo CACNA1H Kit Elisa (A7200)

Mouse CACNA1H ELISA Kit

Mouse CACNA1H ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CACNA1H in tissue homogenates or other biological fluids.

Sandwich (quantitative)

Mouse CACNA1H ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of mouse CACNA1H in mouse. An antibody specific for CACNA1H has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CACNA1H present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CACNA1H Antibody, which binds the captured CACNA1H present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CACNA1H captured in each well. The concentration of CACNA1H can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

Tissue homogenates or other biological fluids.

0.117 ng/ml

0.312-20 ng/ml

1 - 4.5 Hours

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

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