Mouse BAFF ELISA Kit (A517)

Mouse BAFF ELISA Kit

Sandwich (quantitative)

This BAFF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-BAFF Antibody. Standards or samples are then added to the microtire plate wells and BAFF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of BAFF present in the sample, Anti-BAFF Antibody (Biotin) is added to each well to "sandwich" the BAFF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BAFF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 10 pg/ml

46.9-3000 pg/ml

Mouse

Store at 2-8°C for 2 months or at -20°C for 4 months.

B lymphocyte stimulator, B-cell-activating factor, BLYS, CD257, Dendritic cell-derived TNF-like molecule, TALL-1, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4