Scimmia Factor VIII Kit Elisa (A327080)

$764
Spese di spedizione
Tempi di consegna
7-10 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
Monkey Factor VIII ELISA Kit
Descrizione
Monkey Factor VIII ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of monkey Factor VIII in serum, plasma, and other biological fluids.
Tipo di kit
Sandwich (quantitative)
Principio di kit
Monkey Factor VIII ELISA Kit (A327080) employs the sandwich enzyme immunoassay technique for the quantitative measurement of monkey Factor VIII in serum, plasma, and other biological fluids. An antibody specific for Factor VIII has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Factor VIII present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Factor VIII Antibody, which binds the captured Factor VIII present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Factor VIII captured in each well. The concentration of Factor VIII can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Piattaforma
Pre-coated Microplate (12 x 8 well strips)
Tipo di rilevamento
Colormetric
Instrument
Colorimetric Microplate Reader
Tipo di campione
Serum, plasma, and other biological fluids.
Sensibilità
0.19 ng/ml
Campo di rilevamento
0.31-20 ng/ml
Tempo del kit
3h 30m
Reattività
Monkey
Reattività incrociata
No significant cross-reactivity or interference between monkey Factor VIII and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 85% - 95% 90%
EDTA Plasma 8 93% - 109% 99%
Cell Culture Media 8 87% - 102% 93%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 94-108% 92-106% 91-106% 92-102%
EDTA Plasma (n=5) 99-115% 101-117% 97-109% 96-113%
Cell culture media (n=5) 89-101% 89-105% 83-97% 84-95%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Note generali
Information online is representative. Always refer to the Product Manual inside the kit.
Conservazione
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Sinonimi
AHF, Antihemophilic factor, Coagulation factor VIII, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, DXS1253E, F8, F8b, F8c, FA8_HUMAN, factor VIII F8B, Factor VIIIa light chain, FactorVIII, FVIII, Hema, Hemophilia A, Hemophilia, classic, OTTHUMP00000061446, OTTHUMP00000196174, Procoagulant component
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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