Anti-MMP9 Anticorpo [S51-82] (A305026)

Name: Anti-MMP9 Antibody [S51-82]
Brand: Antibodies.com
SKU: A305026
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S51-82] di topo contro MMP9 per WB, IHC, ICC/IF, IP e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-MMP9 Antibody [S51-82]

Descrizione

Mouse monoclonal [S51-82] antibody to MMP9.

Specificità

This antibody detects pro and active forms.

Applicazioni

WB, IHC, ICC/IF, IP, Flow Cytometry

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Immunogeno

Fusion protein amino acids 1-708 (full length) of rat MMP9.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S51-82

Isotipo

IgG2a

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~82 kDa / ~92 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

82 kDa matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, CLG 4B, CLG4B, Collagenase Type 4 beta, Collagenase type IV 92 KD, EC 3.4.24.35, Gelatinase 92 KD, Gelatinase B, Gelatinase beta, GelatinaseB, GELB, Macrophage gelatinase , MANDP2, Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), Matrix Metalloproteinase 9, MMP 9, MMP-9, MMP9_HUMAN, Type V collagenase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Immunofluorescence - Anti-MMP9 Antibody [S51-82] (A305026)

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-MMP9 Antibody [S51-82] (A305026), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) MMP9 Antibody. (D) Composite.

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Figure 2: Western Blot - Anti-MMP9 Antibody [S51-82] (A305026)

Western blot analysis of rat brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Anti-MMP9 Antibody [S51-82] (A305026) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).

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Figure 3: Immunofluorescence - Anti-MMP9 Antibody [S51-82] (A305026)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-MMP9 Antibody [S51-82] (A305026), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) MMP9 Antibody (D) Composite.