Anti-MMP3 Antibody [G05-6A10] (Biotin) (A280494)

Anti-MMP3 Antibody [G05-6A10] (Biotin)

Mouse monoclonal [G05-6A10] antibody to MMP3 (Biotin).

This antibody recognises matrix metalloproteinase-3 (MMP-3) also known as stromelysin-1 (SL-1) and transin-1. MMP-3 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells intima and platelets. MMP-3 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 57 kDa pro-MMP-3. This is then further cleaved by proteolytic enzymes to create the 45 kDa active form. Like other MMP’s, MMP-3 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-3 degrades the collagen proteins, type II, IV, IX as well as the proteoglycans elastin fibronectin and laminin. Through the degradation of these ECM components, growth factors and adhesion molecules, MMP-3 can modify the extracellular environment and modulate cell to cell signaling. As such, MMP-3 is implicated in synaptic plasticity through enzymatic modification of ECM constituents. The biotinylated Mouse anti Human MMP-3 antibody, clone G05-6A10 (MCA6259B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-3 antibody, clone I08-9G7 (MCA6258GA) as the capture antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human partial length of MMP-3 (Phe100-Cys477).

Mouse

Monoclonal

G05-6A10

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human MMP-3 antibody, clone G05-6A10, recognizes matrix metalloproteinase-3 (MMP-3) also known as stromelysin-1 (SL-1) and transin-1. MMP-3 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells intima and platelets (Cui et al. 2017). MMP-3 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 57 kDa pro-MMP-3. This is then further cleaved by proteolytic enzymes to create the 45 kDa active form. Like other MMP’s, MMP-3 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-3 degrades the collagen proteins, type II, IV, IX as well as the proteoglycans elastin fibronectin and laminin (Cui et al. 2017). Through the degradation of these ECM components, growth factors and adhesion molecules, MMP-3 can modify the extracellular environment and modulate cell to cell signaling. As such, MMP-3 is implicated in synaptic plasticity through enzymatic modification of ECM constituents (Lech et al. 2019).The biotinylated Mouse anti Human MMP-3 antibody, clone G05-6A10 (MCA6259B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-3 antibody, clone I08-9G7 (MCA6258GA) as the capture antibody.

Matrix metalloproteinase-3, MMP-3, SL-1, STMY1, Stromelysin-1, Transin-1

This product is for research use only. It is not intended for diagnostic or therapeutic use.