Anti-MLH1 Anticorpo [ARC53543] (A308155)

Name: Anti-MLH1 Antibody [ARC53543]
Brand: Antibodies.com
SKU: A308155
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo monoclonale [ARC53543] di coniglio contro MLH1 per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Garanzia di qualità al 100%

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Tag dei prodotti:

Anticorpo Knockout Convalidato

Descrizione Prodotto

Nome

Anti-MLH1 Antibody [ARC53543]

Descrizione

Rabbit monoclonal [ARC53543] antibody to MLH1.

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:10,000-1:70,000, IHC: 1:50-1:200

Reattività

Human, Mouse

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MLH1 (NP_000240.1).

Sequenza

PGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGD

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC53543

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

85 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC, HNPCC 2, HNPCC2, MGC5172, MLH 1, MLH1_HUMAN, MutL homolog 1, MutL homolog 1 (E. coli), MutL homolog 1 colon cancer nonpolyposis type 2, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1, MutL, E. coli, homolog of, 1

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of various cell lines, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of mouse brain, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts from normal (control) and MLH1 knockout (KO) HeLa cells, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Immunohistochemistry - Anti-MLH1 Antibody [ARC53543] (A308155)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MLH1 expression (negative control sample) using Anti-MLH1 Antibody [ARC53543] (A308155) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.