Anti-MGMT Antibody [MGMT/4791] (A315303)

Anti-MGMT Antibody [MGMT/4791]

Mouse monoclonal [MGMT/4791] antibody to MGMT.

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6 alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

IF, WB, IHC-P

Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).

IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Purified His-tagged MGMT protein.

Mouse

Monoclonal

MGMT/4791

Protein A/G chromatography.

200 µg/ml

21.646 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase