Anti-Methylglyoxal Anticorpo [9E7] (A304733)

Name: Anti-Methylglyoxal Antibody [9E7]
Brand: Antibodies.com
SKU: A304733
Price: 610.0 USD
Availability: InStock

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$610

Anticorpo monoclonale [9E7] di topo contro Methylglyoxal per WB, ICC/IF, IHC, FACS, Flow Cytometry e ELISA.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Methylglyoxal Antibody [9E7]

Descrizione

Mouse monoclonal [9E7] antibody to Methylglyoxal.

Specificità

This antibody is specific to Methylglyoxal modified proteins.

Applicazioni

WB, ICC/IF, IHC, FACS, Flow Cytometry, ELISA

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:50, FACS: 1:50, FCM: 1:50, ELISA: 1:1,000, IHC: 1:50

Reattività

Species Independent

Reattività incrociata

This antibody does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Immunogeno

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH)

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

9E7

Isotipo

IgG2a

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

2 oxo propanal, MG, pyruvaldehyde

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Flow Cytometry - Anti-Methylglyoxal Antibody [9E7] (A304733)

Flow cytometry analysis of human neuroblastoma cells (SH-SY5Y), fixed in 90% methanol, using Anti-Methylglyoxal Antibody [9E7] (A304733) at 1:50 for 30 minutes on ice. The secondary antibody used was Goat Anti-Mouse: PE at 1:100 for 20 minutes at room temperature. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

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Figure 2: Western Blot - Anti-Methylglyoxal Antibody [9E7] (A304733)

Western blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Anti-Methylglyoxal Antibody [9E7] (A304733) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 67 kDa.

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Figure 3: Immunohistochemistry - Anti-Methylglyoxal Antibody [9E7] (A304733)

Immunohistochemistry analysis of rat heart, fixed in formalin. The Primary Antibody used was Anti-Methylglyoxal Antibody [9E7] (A304733) at 1:25 for 3 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X.(A) DAPI (blue) nuclear stain. (B) Actin (C) Methylgyloxal Antibody (D) Composite.

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Figure 4: Immunofluorescence - Anti-Methylglyoxal Antibody [9E7] (A304733)

Immunocytochemistry/Immunofluorescence analysis of human embryonic kidney epithelial cell line (HEK293), fixed in 5% formaldehyde for 5 minutes, using Anti-Methylglyoxal Antibody [9E7] (A304733), at 1:50 for 30-60 min at room temperature. The secondary antibody used was Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at room temperature. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50,000 for 30-60 min at room temperature. Magnification: 20X (2X Zoom). (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite.