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Anti-Mesothelin Anticorpo (A92393)

Nome
Anti-Mesothelin Antibody
Descrizione
Rabbit polyclonal antibody to Mesothelin.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:1,000-1:5,000, IHC: 1:50-1:200
Reattività
Human, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin (NP_037536.2).
Sequenza
EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
46 - 48 kDa / 70 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CAK 1, CAK1, CAK1 antigen, cleaved form, Megakaryocyte potentiating factor, Mesothelin isoform 1 precursor, MPF, Msln, MSLN_HUMAN, Pre pro megakaryocyte potentiating factor, Pre-pro-megakaryocyte-potentiating factor, SMR, SMRP, Soluble MPF mesothelin related protein
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Mesothelin Antibody (A92393) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Mesothelin Antibody (A92393)
Western blot analysis of extracts of OVCAR3 cells, using Anti-Mesothelin Antibody (A92393) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-Mesothelin Antibody (A92393)
Immunohistochemistry analysis of paraffin-embedded human lung cancer (200X) using Anti-Mesothelin Antibody (A92393) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Mesothelin Antibody (A92393)
Immunohistochemistry analysis of paraffin-embedded human lung cancer (400X) using Anti-Mesothelin Antibody (A92393) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Mesothelin Antibody (A92393) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-Mesothelin Antibody (A92393)
Immunohistochemistry analysis of paraffin-embedded human tonsil (200X) using Anti-Mesothelin Antibody (A92393) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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