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MED26 Cell Based Kit Elisa (A103467)

Nome
MED26 Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This MED26 Cell Based ELISA Kit allows for the detection of MED26 and the effects that certain stimulation conditions have on MED26 expression in different cell lines. Qualitative determination of MED26 concentration is achieved by an indirect ELISA format. In essence, the MED26 is captured by Anti-MED26 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MED26 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
Activator recruited cofactor 70 kDa component, Activator-recruited cofactor 70 kDa component, ARC 70, ARC70, Cofactor required for Sp1 transcriptional activation subunit 7, Cofactor required for Sp1 transcriptional activation, subunit 7 (70kD), Cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa, CRSP 7, CRSP complex subunit 7, CRSP7, CRSP70, MED 26, MED26_HUMAN, Mediator complex subunit 26, Mediator of RNA polymerase II transcription subunit 26, Transcriptional coactivator CRSP70
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - MED26 Cell Based ELISA Kit (CB5820)
Western blot analysis of extracts from LOVO cells, using Anti-MED26 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: MED26 Cell Based ELISA Kit (CB5820)
A diagram showing the protocol for MED26 Cell Based ELISA Kit.
Protocol Summary - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: MED26 Cell Based ELISA Kit (CB5820)
A summary of the protocol for MED26 Cell Based ELISA Kit.
Western Blot - Control Antibody - MED26 Cell Based ELISA Kit (CB5820) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Western Blot - MED26 Cell Based ELISA Kit (CB5820)
The mouse monoclonal antibody to GAPDH is used as a positive control inMED26 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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