Anti-MCM3 Antibody [ARC0607] (A306905)

Name: Anti-MCM3 Antibody [ARC0607]
Brand: Antibodies.com
SKU: A306905
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0607] antibody to MCM3 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-MCM3 Antibody [ARC0607]

Description

Rabbit monoclonal [ARC0607] antibody to MCM3.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MCM3 (P25205).

Sequence

MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0607

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA polymerase alpha holoenzyme-associated protein P1, DNA replication licensing factor MCM3, P1-MCM3, p102, RLF subunit beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-MCM3 Antibody [ARC0607] (A306905)

Western blot analysis of extracts of various cell lines, using Anti-MCM3 Antibody [ARC0607] (A306905) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-MCM3 Antibody [ARC0607] (A306905)

Western blot analysis of extracts of Mouse spleen, using Anti-MCM3 Antibody [ARC0607] (A306905) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-MCM3 Antibody [ARC0607] (A306905)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-MCM3 Antibody [ARC0607] (A306905) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-MCM3 Antibody [ARC0607] (A306905)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-MCM3 Antibody [ARC0607] (A306905) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.