Figure 1: Western Blot - Anti-MAP3K8 / COT Antibody (A90597)
Western blot analysis of extracts of 293T cells, using Anti-MAP3K8 / COT Antibody (A90597) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
