Anti-Malondialdehyde Anticorpo [11E3] (A304727)

Name: Anti-Malondialdehyde Antibody [11E3]
Brand: Antibodies.com
SKU: A304727
Price: 610.0 USD
Availability: InStock

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$610

Anticorpo monoclonale [11E3] di topo contro Malondialdehyde per WB, ICC/IF, ELISA e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Malondialdehyde Antibody [11E3]

Descrizione

Mouse monoclonal [11E3] antibody to Malondialdehyde.

Specificità

This antibody is specific to Malondialdehyde conjugated proteins.

Applicazioni

WB, ICC/IF, ELISA, IHC

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:50, ELISA: 1:1,000, IHC: 1:100

Reattività

Species Independent

Reattività incrociata

This antibody does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.

Immunogeno

Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH)

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

11E3

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

1133 Tetramethoxypropane, MDA

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-Malondialdehyde Antibody [11E3] (A304727)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Malondialdehyde protein using Anti-Malondialdehyde Antibody [11E3] (A304727) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature.

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Figure 2: Western Blot - Anti-Malondialdehyde Antibody [11E3] (A304727)

Western blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Anti-Malondialdehyde Antibody [11E3] (A304727) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 µg). Lane 3: Malondialdehyde-BSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 67 kDa.

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Figure 3: Immunofluorescence - Anti-Malondialdehyde Antibody [11E3] (A304727)

Immunocytochemistry/Immunofluorescence analysis of human embryonic kidney epithelial cell line (HEK293), fixed in 5% formaldehyde for 5 minutes, using Anti-Malondialdehyde Antibody [11E3] (A304727), at 1:50 for 30-60 min at room temperature. The secondary antibody used was Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at room temperature. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50,000 for 30-60 min at room temperature. Magnification: 20X (2X Zoom). (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite.

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Figure 4: Immunohistochemistry - Anti-Malondialdehyde Antibody [11E3] (A304727)

Immunohistochemistry analysis of mouse kidney. The Primary Antibody used was Anti-Malondialdehyde Antibody [11E3] (A304727) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 10X.