Anti-MAdCAM1 Antibody [314G8] - BSA and Azide free
Descrizione
Mouse monoclonal [314G8] antibody to MAdCAM1.
Specificità
This antibody recognises human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.
Applicazioni
ELISA, Functional Assays
Diluizioni consigliate
Flow Cytometry: 1:10 - 1:100, IHC-Fr: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reattività
Human
Immunogeno
Recombinant soluble MAdCAM-1-fc fusion protein.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
314G8
Isotipo
IgG1
Coniugare
Unconjugated
Purificazione
Protein A affinity chromatography of tissue culture supernatant.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline.
Conservazione
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
Note generali
Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004).
