Anti-LGI1 Anticorpo [S283-7] (A305011)

Name: Anti-LGI1 Antibody [S283-7]
Brand: Antibodies.com
SKU: A305011
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S283-7] di topo contro LGI1 per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-LGI1 Antibody [S283-7]

Descrizione

Mouse monoclonal [S283-7] antibody to LGI1.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S283-7

Isotipo

IgG2a

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~60 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

ADLTE, ADPAEF, ADPEAF, EPITEMPIN, Epitempin 1, Epitempin-1, EPT, ETL1, IB1099, leucine rich glioma inactivated 1, Leucine rich glioma-inactivated protein 1, Leucine-rich glioma-inactivated protein 1, LGI1_HUMAN, OTTHUMP00000020121, OTTHUMP00000020122

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Immunofluorescence - Anti-LGI1 Antibody [S283-7] (A305011)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-LGI1 Antibody [S283-7] (A305011), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LGI1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-LGI1 Antibody [S283-7] (A305011)

Western blot analysis of rat brain membrane showing detection of ~60 kDa LGI1 protein using Anti-LGI1 Antibody [S283-7] (A305011) at 1:1,000 for 1 hour at room temperature. Load: 10 µg. The secondary antibody used was Goat Anti-Mouse HRP at 1:200 for 1 hour at room temperature. Predicted/Observed Size: ~60 kDa.

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Figure 3: Immunofluorescence - Anti-LGI1 Antibody [S283-7] (A305011)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-LGI1 Antibody [S283-7] (A305011), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LGI1 Antibody. (D) Composite.