Anti-LEF1 Antibody (A89721)

Name: Anti-LEF1 Antibody
Brand: Antibodies.com
SKU: A89721
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to LEF1 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-LEF1 Antibody

Description

Rabbit polyclonal antibody to LEF1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IP: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-132 of human LEF1 (NP_057353.1).

Sequence

TDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

25 - 58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-LEF1 Antibody (A89721)

Western blot analysis of extracts of various cell lines, using Anti-LEF1 Antibody (A89721) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunofluorescence - Anti-LEF1 Antibody (A89721)

Immunofluorescence analysis of mouse thymus cells using Anti-LEF1 Antibody (A89721) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-LEF1 Antibody (A89721)

Immunofluorescence analysis of rat thymus cells using Anti-LEF1 Antibody (A89721) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-LEF1 Antibody (A89721)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-LEF1 Antibody (A89721). This Western blot was performed on the immunoprecipitate using Anti-LEF1 Antibody (A89721) at a dilution of 1:1000.

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