Anti-Lck (phospho Tyr394) Antibody (A12463)

Name: Anti-Lck (phospho Tyr394) Antibody
Brand: Antibodies.com
SKU: A12463
Price: 485.0 USD
Availability: InStock

Enlarge Image

$485

Rabbit polyclonal antibody to Lck (phospho Tyr394) for WB.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Plasma Membrane Marker

Specifications

Name

Anti-Lck (phospho Tyr394) Antibody

Description

Rabbit polyclonal antibody to Lck (phospho Tyr394).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around Y394 of human LCK (NP_005347.3).

Sequence

NEYTA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Enlarge Image

Western Blot - Anti-Lck (phospho Tyr394) Antibody (A12463)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Lck (phospho Tyr394) Antibody (A12463) at 1:1,000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Western Blot - Anti-Lck (phospho Tyr394) Antibody (A12463)

Western blot analysis of extracts of Jurkat cells, using Anti-Lck (phospho Tyr394) Antibody (A12463) at 1:1,000 dilution. Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Publishing research using Anti-Lck (phospho Tyr394) Antibody (A12463)? Please let us know so that we can list the citation on this page.