Anti-Lactate Dehydrogenase Aptamer [pL2] (Biotin) (A320884)

Spese di spedizione
Tempi di consegna
30-45 Giorni lavorativi
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+1 (314) 370-6046
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Name
Anti-Lactate Dehydrogenase Aptamer [pL2] (Biotin)
Description
Synthetic DNA aptamer to Lactate Dehydrogenase [pL2] (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: 20mM Tris-HCl Buffer, pH 8, with 50mM Sodium Chloride, 5mM Potassium Chloride, and 5mM Magnesium Chloride. Binding Temperature: 25°C.
Sequence
5'-dGpdApdApdCpdTpdCpdApdTpdTpdGpdGpdCpdTpdGpdGpdApdGpdGpdCpdGpdGpdCpdApdGpdTpdApdCpdCpdGpdCpdTpdTpdGpdApdGpdTpdTpdCp-3'
Aptamer Length
38 nt
Aptamer Molecular Weight (Da)
11,750.66 Da
Aptamer Affinity
Affinity (Kd) = 49.6 nM
Extinction Coefficient
358500 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Lactate Dehydrogenase Aptamer [pL2] (Biotin) (A320884) is a replica of the aptamer described in PMID: 22459583.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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