Figure 1: Western Blot - Anti-Kinetochore Antibody (A88881)
Western blot analysis of extracts of 293T cells, using Anti-Kinetochore Antibody (A88881) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
