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Anti-KIFAP3 Anticorpo (A14579)

Nome
Anti-KIFAP3 Antibody
Descrizione
Rabbit polyclonal antibody to KIFAP3.
Applicazioni
WB, ICC/IF, IP
Diluizioni consigliate
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:50-1:100
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KIFAP3 (NP_055785.2).
Sequenza
MQGEDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYI
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
110 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
FLJ22818, KAP 3, KAP-3, KAP3, KIF3AP, KIFA3_HUMAN, Kinesin associated protein 3, Kinesin-associated protein 3, SMAP, Smg GDS, Smg GDS associated protein, Smg GDS-associated protein
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-KIFAP3 Antibody (A14579) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-KIFAP3 Antibody (A14579)
Western blot analysis of extracts of various cell lines, using Anti-KIFAP3 Antibody (A14579) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-KIFAP3 Antibody (A14579) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunofluorescence - Anti-KIFAP3 Antibody (A14579)
Immunofluorescence analysis of U2OS cells using Anti-KIFAP3 Antibody (A14579) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-KIFAP3 Antibody (A14579) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-KIFAP3 Antibody (A14579)
Immunofluorescence analysis of L929 cells using Anti-KIFAP3 Antibody (A14579) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-KIFAP3 Antibody (A14579) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-KIFAP3 Antibody (A14579)
Immunofluorescence analysis of H9C2 cells using Anti-KIFAP3 Antibody (A14579) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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