Rabbit polyclonal antibody to Keratin 18 (phospho Ser52).
Specificità
This antibody detects endogenous levels of Keratin 18 only when phosphorylated at Ser52.
Applicazioni
WB, IF, ELISA
Diluizioni consigliate
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000
Reattività
Human
Immunogeno
Synthetic peptide derived from human Keratin 18 around the phosphorylation site of Ser52 (amino acids 21-70).
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Peso molecolare
48kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CK18, CK19, CK8, K18, K19, K8, keratin 18, keratin 19, keratin 8, Keratin type I 40kDa, Keratin type I cytoskeletal 18, Keratin type I cytoskeletal 19, Keratin type II cytoskeletal 8, KRT18, KRT19, KRT8
Figure 1: Western Blot - Anti-Keratin 18 (phospho Ser52) Antibody (A94696)
Western blot analysis of lysates from HepG2 cells using Anti-Keratin 18 (phospho Ser52) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Keratin 18 (phospho Ser52) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human brain using Anti-Keratin 18 (phospho Ser52) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Prodotti alternativi a Anti-Keratin 18 (phospho Ser52) Anticorpo (A94696)
