Anti-Keap1 Antibody (A83585)

Name: Anti-Keap1 Antibody
Brand: Antibodies.com
SKU: A83585
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to Keap1 for ELISA, IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Keap1 Antibody

Description

Goat polyclonal antibody to Keap1.

Applications

ELISA, IF, IHC

Dilutions

ELISA: 1:32,000, IHC: 3-5µg/ml, IF: 5µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region (near N terminus), amino acids 41-53, of human Keap1.

Sequence

C-EVTPSQHGNRTFS

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cytosolic inhibitor of Nrf2, INRF2, Kelch-like ECH-associated protein 1, Kelch-like protein 19, KIAA0132, KLHL19

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunofluorescence - Anti-Keap1 Antibody (A83585)

Keap1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Keap1 Antibody (A83585) at 5µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 5µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Immunohistochemistry - Anti-Keap1 Antibody (A83585)

Keap1 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Keap1 Antibody (A83585) at 3.8µg/ml and revealed with alkaline phosphatase (AP).

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